Bars show the mean and standard deviation. In maximum growth rate plots, each symbol is a single biological replicate. In growth curve plots the solid line is the mean OD 600 of three biological replicates, with dotted lines showing the standard deviation. Synthetic biosensors for precise gene control and real-time monitoring of metabolites. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. In Strain Engineering: Methods and Protocols (ed. Improving lambda Red genome engineering in Escherichia coli via rational removal of endogenous nucleases. Scaling computation and memory in living cells. Permanent genetic memory with >1-byte capacity.
#Gene expression c artoon serial
The serial cultivation of human diploid cell strains. Procedures for detecting outlying observations in samples. Escherichia coli “Marionette” strains with 12 highly optimized small-molecule sensors. RecJ exonuclease: substrates, products and interaction with SSB. Characterization of cell death in Escherichia coli mediated by XseA, a large subunit of exonuclease VII. Structure and biosynthesis of unbranched multicopy single-stranded DNA by reverse transcriptase in a clinical Eschehchia coli isolate. Isolation and characterization of the gene encoding yeast debranching enzyme. Processing and integration of functionally oriented prespacers in the Escherichia coli CRISPR system depends on bacterial host exonucleases. Ramachandran, A., Summerville, L., Learn, B. Selective loading and processing of prespacers for precise CRISPR adaptation. Rewritable digital data storage in live cells via engineered control of recombination directionality. Direct CRISPR spacer acquisition from RNA by a natural reverse transcriptase–Cas1 fusion protein. Reverse transcriptase in a clinical strain of Escherichia coli: production of branched RNA-linked msDNA. Bacterial retrons encode tripartite toxin/antitoxin systems. Bacterial retrons function in anti-phage defense. Structural and mechanistic basis of PAM-dependent spacer acquisition in CRISPR–Cas systems. Cas1–Cas2 complex formation mediates spacer acquisition during CRISPR–Cas adaptive immunity. Proteins and DNA elements essential for the CRISPR adaptation process in Escherichia coli. Genomically encoded analog memory with precise in vivo DNA writing in living cell populations. Precise genome editing across kingdoms of life using retron-derived DNA. High-throughput functional variant screens via in vivo production of single-stranded DNA. Functional genetic variants revealed by massively parallel precise genome editing. Retroelement-based genome editing and evolution. Molecular recordings by directed CRISPR spacer acquisition. Recording of elapsed time and temporal information about biological events using Cas9. Continuous genetic recording with self-targeting CRISPR–Cas in human cells. Slingshot: cell lineage and pseudotime inference for single-cell transcriptomics. Lineage tracing meets single-cell omics: opportunities and challenges. Transcriptional recording by CRISPR spacer acquisition from RNA. Multiplex recording of cellular events over time on CRISPR biological tape. Synthetic recombinase-based state machines in living cells. Molecular digital data storage using DNA. Robust direct digital-to-biological data storage in living cells.
#Gene expression c artoon movie
CRISPR–Cas encoding of a digital movie into the genomes of a population of living bacteria. Next-generation digital information storage in DNA. CRISPR provides acquired resistance against viruses in prokaryotes. Retrons and their applications in genome engineering.
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